Staining:Article Title: p38-mediated cell growth and survival drive rapid embryonic wound repair.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit anti phospho-p38 (Thr180/Tyr182) (1:20 for staining, 1:2000 for Western) Cell Signaling Technology Cat# 9215; RRID:AB_331762 mouse anti-alpha-tubulin (1:1000) Sigma-Aldrich Cat#: T6199; RRID: AB_477583 goat anti-rabbit IgG horseradish peroxidase conjugated (1:10,000) Jackson ImmunoResearch Laboratories Cat# 111-035-003; RRID:AB_2313567 donkey anti-mouse IgG horseradish peroxidase conjugated (1:10,000) Jackson ImmunoResearch Laboratories Cat#: 715-035-150; RRID: AB_2340770 rabbit anti-phospho-NKCC1 (Thr212/Thr217) (1:20) Sigma-Aldrich Cat# AV43805; RRID:AB_1854501 mouse anti-GFP (1:50) Invitrogen Cat# A-11120; RRID:AB_221568 goat anti-rabbit Alexa 568 (1:500) Invitrogen Cat# A-11011; RRID:AB_143157 goat anti-mouse Alexa 488 (1:500) Invitrogen Cat# A-21121; RRID:AB_2535764 phalloidin Alexa 647 (1:500) Invitrogen Cat# A22287; RRID:AB_2620155 Chemicals, peptides, and recombinant proteins SB203580 Enzo Life Sciences, Inc Cat#: BML-EI286 Anisomycin Tocris Bioscience Cat#: 1290 Cycloheximide Abcam Cat#: ab120093 Puromycin Sigma Cat#: P9620 EUK134 Sigma Cat#: SML0743 Bumetanide Sigma Cat#: B3023 Azosemide TRC Canada Cat#: A965145 Aldosterone Sigma Cat#: A9477 H2DCFDA Thermo-Fisher Scientific Cat#: D6883 Critical commercial assays MEGAscript T7 Transcription Kit Thermo-Fisher Scientific Cat#: AMB1334 Experimental models: Organisms/strains D. melanogaster: endo-E-cadherin:tdTomato Huang et al., 2009 RRID:BDSC_58789 D. melanogaster: sqh–sqh:GFP Royou et al., 2004 RRID:BDSC_57145 D. melanogaster: sqh-sqh:mCherry Martin et al., 2009 RRID:BDSC_59024 D. melanogaster: zip-zip:GFP Buszczak et al., 2007 N/A D. melanogaster: sqh-utrophinABD:GFP Rauzi et al., 2010 N/A D. melanogaster: resille:GFP Buszczak et al., 2007 N/A D. melanogaster: endo-DE-cadherin:GFP Huang et al., 2009 RRID:BDSC_60584 D. melanogaster: b-catenin-b-catenin:GFP McCartney et al., 2001 N/A D. melanogaster: p38a1 Craig et al., 2004 RRID:BDSC_8822 D. melanogaster: yellow white N/A N/A D. melanogaster: UAS-GFP N/A RRID:BDSC_5431 D. melanogaster: UAS-lic (Bischof et al., 2013) FlyORF #F001674 D. melanogaster: UAS-ncc69 (Leiserson et al., 2011) N/A D. melanogaster: UAS-p38b-DN N/A RRID:BDSC_59005 D. melanogaster: UAS-basket:GFP (Jiang et al., 2019). ..
Western Blot:Article Title: p38-mediated cell growth and survival drive rapid embryonic wound repair.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit anti phospho-p38 (Thr180/Tyr182) (1:20 for staining, 1:2000 for Western) Cell Signaling Technology Cat# 9215; RRID:AB_331762 mouse anti-alpha-tubulin (1:1000) Sigma-Aldrich Cat#: T6199; RRID: AB_477583 goat anti-rabbit IgG horseradish peroxidase conjugated (1:10,000) Jackson ImmunoResearch Laboratories Cat# 111-035-003; RRID:AB_2313567 donkey anti-mouse IgG horseradish peroxidase conjugated (1:10,000) Jackson ImmunoResearch Laboratories Cat#: 715-035-150; RRID: AB_2340770 rabbit anti-phospho-NKCC1 (Thr212/Thr217) (1:20) Sigma-Aldrich Cat# AV43805; RRID:AB_1854501 mouse anti-GFP (1:50) Invitrogen Cat# A-11120; RRID:AB_221568 goat anti-rabbit Alexa 568 (1:500) Invitrogen Cat# A-11011; RRID:AB_143157 goat anti-mouse Alexa 488 (1:500) Invitrogen Cat# A-21121; RRID:AB_2535764 phalloidin Alexa 647 (1:500) Invitrogen Cat# A22287; RRID:AB_2620155 Chemicals, peptides, and recombinant proteins SB203580 Enzo Life Sciences, Inc Cat#: BML-EI286 Anisomycin Tocris Bioscience Cat#: 1290 Cycloheximide Abcam Cat#: ab120093 Puromycin Sigma Cat#: P9620 EUK134 Sigma Cat#: SML0743 Bumetanide Sigma Cat#: B3023 Azosemide TRC Canada Cat#: A965145 Aldosterone Sigma Cat#: A9477 H2DCFDA Thermo-Fisher Scientific Cat#: D6883 Critical commercial assays MEGAscript T7 Transcription Kit Thermo-Fisher Scientific Cat#: AMB1334 Experimental models: Organisms/strains D. melanogaster: endo-E-cadherin:tdTomato Huang et al., 2009 RRID:BDSC_58789 D. melanogaster: sqh–sqh:GFP Royou et al., 2004 RRID:BDSC_57145 D. melanogaster: sqh-sqh:mCherry Martin et al., 2009 RRID:BDSC_59024 D. melanogaster: zip-zip:GFP Buszczak et al., 2007 N/A D. melanogaster: sqh-utrophinABD:GFP Rauzi et al., 2010 N/A D. melanogaster: resille:GFP Buszczak et al., 2007 N/A D. melanogaster: endo-DE-cadherin:GFP Huang et al., 2009 RRID:BDSC_60584 D. melanogaster: b-catenin-b-catenin:GFP McCartney et al., 2001 N/A D. melanogaster: p38a1 Craig et al., 2004 RRID:BDSC_8822 D. melanogaster: yellow white N/A N/A D. melanogaster: UAS-GFP N/A RRID:BDSC_5431 D. melanogaster: UAS-lic (Bischof et al., 2013) FlyORF #F001674 D. melanogaster: UAS-ncc69 (Leiserson et al., 2011) N/A D. melanogaster: UAS-p38b-DN N/A RRID:BDSC_59005 D. melanogaster: UAS-basket:GFP (Jiang et al., 2019). ..
Recombinant:Article Title: p38-mediated cell growth and survival drive rapid embryonic wound repair.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit anti phospho-p38 (Thr180/Tyr182) (1:20 for staining, 1:2000 for Western) Cell Signaling Technology Cat# 9215; RRID:AB_331762 mouse anti-alpha-tubulin (1:1000) Sigma-Aldrich Cat#: T6199; RRID: AB_477583 goat anti-rabbit IgG horseradish peroxidase conjugated (1:10,000) Jackson ImmunoResearch Laboratories Cat# 111-035-003; RRID:AB_2313567 donkey anti-mouse IgG horseradish peroxidase conjugated (1:10,000) Jackson ImmunoResearch Laboratories Cat#: 715-035-150; RRID: AB_2340770 rabbit anti-phospho-NKCC1 (Thr212/Thr217) (1:20) Sigma-Aldrich Cat# AV43805; RRID:AB_1854501 mouse anti-GFP (1:50) Invitrogen Cat# A-11120; RRID:AB_221568 goat anti-rabbit Alexa 568 (1:500) Invitrogen Cat# A-11011; RRID:AB_143157 goat anti-mouse Alexa 488 (1:500) Invitrogen Cat# A-21121; RRID:AB_2535764 phalloidin Alexa 647 (1:500) Invitrogen Cat# A22287; RRID:AB_2620155 Chemicals, peptides, and recombinant proteins SB203580 Enzo Life Sciences, Inc Cat#: BML-EI286 Anisomycin Tocris Bioscience Cat#: 1290 Cycloheximide Abcam Cat#: ab120093 Puromycin Sigma Cat#: P9620 EUK134 Sigma Cat#: SML0743 Bumetanide Sigma Cat#: B3023 Azosemide TRC Canada Cat#: A965145 Aldosterone Sigma Cat#: A9477 H2DCFDA Thermo-Fisher Scientific Cat#: D6883 Critical commercial assays MEGAscript T7 Transcription Kit Thermo-Fisher Scientific Cat#: AMB1334 Experimental models: Organisms/strains D. melanogaster: endo-E-cadherin:tdTomato Huang et al., 2009 RRID:BDSC_58789 D. melanogaster: sqh–sqh:GFP Royou et al., 2004 RRID:BDSC_57145 D. melanogaster: sqh-sqh:mCherry Martin et al., 2009 RRID:BDSC_59024 D. melanogaster: zip-zip:GFP Buszczak et al., 2007 N/A D. melanogaster: sqh-utrophinABD:GFP Rauzi et al., 2010 N/A D. melanogaster: resille:GFP Buszczak et al., 2007 N/A D. melanogaster: endo-DE-cadherin:GFP Huang et al., 2009 RRID:BDSC_60584 D. melanogaster: b-catenin-b-catenin:GFP McCartney et al., 2001 N/A D. melanogaster: p38a1 Craig et al., 2004 RRID:BDSC_8822 D. melanogaster: yellow white N/A N/A D. melanogaster: UAS-GFP N/A RRID:BDSC_5431 D. melanogaster: UAS-lic (Bischof et al., 2013) FlyORF #F001674 D. melanogaster: UAS-ncc69 (Leiserson et al., 2011) N/A D. melanogaster: UAS-p38b-DN N/A RRID:BDSC_59005 D. melanogaster: UAS-basket:GFP (Jiang et al., 2019). ..
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